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Image Search Results


Journal: EMBO Reports

Article Title: PARK15 / FBXO7 is dispensable for PINK1 /Parkin mitophagy in iNeurons and HeLa cell systems

doi: 10.15252/embr.202256399

Figure Lengend Snippet:

Article Snippet: SpCas9 and AsCas12a/AsCpf1 expression plasmids pET‐NLS‐Cas9‐6xHis (Addgene plasmid # 62934) and modified pDEST‐his‐AsCpf1‐EC, generated by deleting the MBP sequence from plasmid pDEST‐hisMBP‐AsCpf1‐EC (Addgene plasmid # 79007), were transformed into RosettaTM(DE3)pLysS Competent Cells (Novagen), respectively, for expression.

Techniques: Recombinant, Plasmid Preparation, Sequencing, Membrane, Electron Microscopy, Staining, Western Blot, Protease Inhibitor, Peptide Fractionation, Bicinchoninic Acid Protein Assay, Software, Mass Spectrometry, Transfection, Imaging